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nitrocellulose membranes  (Bio-Rad)


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    Structured Review

    Bio-Rad nitrocellulose membranes
    Nitrocellulose Membranes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1302 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transfer+to+nitrocellulose/Trans-Blot+Turbo+Mini+Nitrocellulose+Transfer+Packs/pmc13140060-235-24-26
    Average 96 stars, based on 1302 article reviews
    nitrocellulose membranes - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Unveiling the molecular identity of plant autophagic compartments: A proteo-lipidomic study in Arabidopsis thaliana
    Article Snippet: Proteins were denaturated at 55 °C for 15 min. After migration on 12% SDS-PAGE (TGX Stain-Free FastCast Acrylamide kit, BioRad), equal amount of protein per lane was verified by stain-free activation of the loaded gel. .. After transfer to nitrocellulose (BioRad, #1704270), membranes were incubated with an anti-GFP mouse antibody (Roche, #11814460001, 1/2000). .. Peroxidase activity coupled to the Goat anti-Mouse antibody at a dilution of 1/10000 (BioRad, #1706516) was revealed using Wester Lighting Plus-ECL (BioRad) and detected with the Chemidoc MP Imaging system (BioRad).

    Article Title: Phosphatidylinositol-4-phosphate controls autophagosome formation in Arabidopsis thaliana
    Article Snippet: Proteins were denaturated at 55 °C for 15 min. After migration on 12% SDS-PAGE (TGX Stain-Free FastCast Acrylamide kit, BioRad), equal protein quantity per lane was verified by stain-free activation of the loaded gel. .. After transfer to nitrocellulose (BioRad, #1704270), membranes were incubated with an anti-GFP mouse antibody (Roche, #11814460001) at a dilution of 1/5000. .. Peroxidase activity coupled to the Goat anti-Mouse antibody at a dilution of 1/5000 (Bio-Rad, #1706516) was revealed using Wester Lighting Plus-ECL (PerkinElmer) and detected with the Chemidoc MP Imaging system (Bio-Rad).

    Article Title: The cyto-linker and scaffolding protein "plectin" mis-localization leads to softening of cancer cells.
    Article Snippet: Protein concentrations were determined by the Pierce 660 nm protein-assay (Thermo Fisher) and the indicated amounts of total protein were loaded on a 12% PAA gel. .. After transfer to nitrocellulose (Trans-Blot Turbo, Biorad) the equal loading was controlled with Ponceau S (Thermo Fisher) and the membrane was blocked in 1% BSA in PBS for 30 min. Primary antibodies (mouse-anti-a-tubulin (clone DM1A, 1 : 5000) and mouse-anti-actin (clone AC-40, 1 : 2000), both from Sigma Aldrich) were added and incubated for 1 hour at room temperature. .. For the detection of the bound antibodies goat anti-mouse-IgG-Cy 5 (Amersham, 1 : 5000) was added and incubated again for 1 hour at room temperature.

    Article Title: A dual component system instructs membrane hydrolysis during the final stages of plant autophagy
    Article Snippet: After migration on 12% SDS-PAGE (TGX Stain-Free FastCast Acrylamide kit, BioRad), equal protein quantity per lane was verified by stain-free activation of the loaded gel. .. After transfer to nitrocellulose (BioRad, #1704270), membranes were incubated with an anti-GFP mouse antibody (Roche, #11814460001) at a dilution of 1/10,000 for 35S:GFP-ATG8A expressing plants ( Fig. S6, S9 ). ..

    Bicinchoninic Acid Protein Assay:

    Article Title: A truncated derivative of FGFR1 kinase cooperates with FLT3 and KIT to transform hematopoietic stem cells in syndromic and de novo AML
    Article Snippet: For Western blotting, cells were lysed in ice-cold T-PERTM Tissue Extraction Reagent (Thermo Scientific), 0.5 M EDTA, and a mixture of proteases inhibitors. .. 20 μg protein samples were measured using the PierceTM BCA protein Assay kit and analyzed by SDS-PAGE followed by transfer to nitrocellulose (Bio-Rad) membrane using the Bio-Rad gel and transfer apparatus. ..

    SDS Page:

    Article Title: A truncated derivative of FGFR1 kinase cooperates with FLT3 and KIT to transform hematopoietic stem cells in syndromic and de novo AML
    Article Snippet: For Western blotting, cells were lysed in ice-cold T-PERTM Tissue Extraction Reagent (Thermo Scientific), 0.5 M EDTA, and a mixture of proteases inhibitors. .. 20 μg protein samples were measured using the PierceTM BCA protein Assay kit and analyzed by SDS-PAGE followed by transfer to nitrocellulose (Bio-Rad) membrane using the Bio-Rad gel and transfer apparatus. ..

    Membrane:

    Article Title: A truncated derivative of FGFR1 kinase cooperates with FLT3 and KIT to transform hematopoietic stem cells in syndromic and de novo AML
    Article Snippet: For Western blotting, cells were lysed in ice-cold T-PERTM Tissue Extraction Reagent (Thermo Scientific), 0.5 M EDTA, and a mixture of proteases inhibitors. .. 20 μg protein samples were measured using the PierceTM BCA protein Assay kit and analyzed by SDS-PAGE followed by transfer to nitrocellulose (Bio-Rad) membrane using the Bio-Rad gel and transfer apparatus. ..

    Article Title: The cyto-linker and scaffolding protein "plectin" mis-localization leads to softening of cancer cells.
    Article Snippet: Protein concentrations were determined by the Pierce 660 nm protein-assay (Thermo Fisher) and the indicated amounts of total protein were loaded on a 12% PAA gel. .. After transfer to nitrocellulose (Trans-Blot Turbo, Biorad) the equal loading was controlled with Ponceau S (Thermo Fisher) and the membrane was blocked in 1% BSA in PBS for 30 min. Primary antibodies (mouse-anti-a-tubulin (clone DM1A, 1 : 5000) and mouse-anti-actin (clone AC-40, 1 : 2000), both from Sigma Aldrich) were added and incubated for 1 hour at room temperature. .. For the detection of the bound antibodies goat anti-mouse-IgG-Cy 5 (Amersham, 1 : 5000) was added and incubated again for 1 hour at room temperature.

    Western Blot:

    Article Title: Palmitoylation-dependent regulation of cardiomyocyte Rac1 signaling activity and minor effects on cardiac hypertrophy
    Article Snippet: .. Eluted proteins are run on SDS gels followed by transfer to nitrocellulose (Bio-Rad 162-0112) and Western blotting. ..

    Article Title: Palmitoylation-dependent regulation of cardiomyocyte Rac1 signaling activity and minor effects on cardiac hypertrophy.
    Article Snippet: Received for publication, July 10, 2023, and in revised form, September 28, 2023 Published, Papers in Press, November 3, 2023, https://doi.org/10.1016/j.jbc.2023.105426 Tanya A. Baldwin, James P. Teuber , Yasuhide Kuwabara , Araskumar Subramani , Suh-Chin J. Lin , Onur Kanisicak, Ronald J. Vagnozzi , Weiqi Zhang, Matthew J. Brody* , and Jeffery D. Molkentin* From the Department of Pediatrics, Cincinnati Children’s Hospital Medical Center, Cincinnati, Ohio, USA; Department of Pharmacology, University of Michigan, Ann Arbor, Michigan, USA; Department of Pathology, University of Cincinnati, Cincinnati, Ohio, USA; Division of Cardiology, Department of Medicine, Consortium for Fibrosis Research & Translation, University of Colorado Anschutz Medical Campus, Aurora, Colorado, USA; Laboratory of Molecular Psychiatry, Department of Mental Health, University of Münster, Münster, Germany; Division of Cardiovascular Medicine, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA

    Expressing:

    Article Title: A dual component system instructs membrane hydrolysis during the final stages of plant autophagy
    Article Snippet: After migration on 12% SDS-PAGE (TGX Stain-Free FastCast Acrylamide kit, BioRad), equal protein quantity per lane was verified by stain-free activation of the loaded gel. .. After transfer to nitrocellulose (BioRad, #1704270), membranes were incubated with an anti-GFP mouse antibody (Roche, #11814460001) at a dilution of 1/10,000 for 35S:GFP-ATG8A expressing plants ( Fig. S6, S9 ). ..



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